Fluid phase biopsy:循環血中血管内皮細胞同定で心筋梗塞診断

HD-CEC (High-Definition Circulating Endothelial Cell) assay

心筋梗塞を含む病的状況で循環中血管内皮細胞(CECs)が増加する。これをfluid phase
biopsy technologyプラットフォームにて、例えば、がん細胞を検がなされている。

心筋梗塞患者で、DAPI、 CD146  von Willebrand Factor の陽性シグナルと、CD45陰性シグナルを示す細胞を、CECｓ陽性と定義し検出する。

Study subjects	BCT	# of blood draws	Mean CEC ml−1	Median CEC ml−1	Quartiles lower	CEC ml−1 upper
Myocardial infraction	EDTA	51	50.3	20.5	6.3	53.1
Myocardial infraction	Streck	28	23.0	4.4	1.5	24.6
Myocardial infraction	ALL	79	40.6	13.7	2.9	39.4
Healthy control	EDTA	20	0.2	0.0	0	0
Healthy control	Streck	14	0.4	0.2	0	0.5
Healthy control	ALL	34	0.3	0.0	0	0.5
Vascular surgery	EDTA	6	0.00	0.0	0	0

Fluid phase biopsy for detection and characterization of circulating endothelial cells in myocardial infarction
Kelly Bethel et al
2014 Phys. Biol. 11 016002
doi:10.1088/1478-3975/11/1/016002



Figure 1. Typical circulating endothelial cell detected with the HD-CEC assay. The panels show (a) a DAPI positive, large nucleus surrounded by numerous smaller nuclei of WBCs, (b) CD45 positivity on white blood cells only, (c) a CD146 positive cytoplasm, and (d) vWF positive cytoplasm with a staining pattern that is distinct from the CD146. Notice that surrounding platelets are also positive for vWF. Panel (e) is the composite image of DAPI, CD45 and CD146 and panel (f) is the composite image of DAPI, CD45 and vWF.