心筋梗塞を含む病的状況で循環中血管内皮細胞(CECs)が増加する。これをfluid phase
biopsy technologyプラットフォームにて、例えば、がん細胞を検がなされている。
心筋梗塞患者で、DAPI、 CD146 von Willebrand Factor の陽性シグナルと、CD45陰性シグナルを示す細胞を、CECｓ陽性と定義し検出する。
|
Study subjects
|
BCT
|
# of blood draws
|
Mean CEC ml−1
|
Median CEC ml−1
|
Quartiles lower
|
CEC ml−1 upper
|
|
Myocardial infraction
|
EDTA
|
51
|
50.3
|
20.5
|
6.3
|
53.1
|
|
Myocardial infraction
|
Streck
|
28
|
23.0
|
4.4
|
1.5
|
24.6
|
|
Myocardial infraction
|
ALL
|
79
|
40.6
|
13.7
|
2.9
|
39.4
|
|
Healthy control
|
EDTA
|
20
|
0.2
|
0.0
|
0
|
0
|
|
Healthy control
|
Streck
|
14
|
0.4
|
0.2
|
0
|
0.5
|
|
Healthy control
|
ALL
|
34
|
0.3
|
0.0
|
0
|
0.5
|
|
Vascular surgery
|
EDTA
|
6
|
0.00
|
0.0
|
0
|
0
|
Fluid phase biopsy for detection and characterization of circulating endothelial cells in myocardial infarction
Kelly Bethel et al
2014 Phys. Biol. 11 016002
doi:10.1088/1478-3975/11/1/016002
Figure 1. Typical circulating endothelial cell detected with the HD-CEC assay. The panels show (a) a DAPI positive, large nucleus surrounded by numerous smaller nuclei of WBCs, (b) CD45 positivity on white blood cells only, (c) a CD146 positive cytoplasm, and (d) vWF positive cytoplasm with a staining pattern that is distinct from the CD146. Notice that surrounding platelets are also positive for vWF. Panel (e) is the composite image of DAPI, CD45 and CD146 and panel (f) is the composite image of DAPI, CD45 and vWF.
